polymerase chain reaction

English - Turkish

Definition of polymerase chain reaction in English Turkish dictionary

(Biyoloji) Polimeraz zincir tepkimesi

English - English

Definition of polymerase chain reaction in English English dictionary

a technique in molecular biology for creating multiple copies of DNA from a sample; used in genetic fingerprinting etc

laboratory technique used to replicate segments of DNA by repeatedly splitting the DNA strands and duplicating them with a DNA polymerase enzyme, PCR (Molecular Biology)

A technique for amplifying DNA sequences in vitro by separating the DNA into two strands and incubating it with oligonucleotide primers and DNA polymerase. It can amplify a specific sequence of DNA by as many as one billion times and is important in biotechnology, forensics, medicine, and genetic research. Laboratory technique used to make numerous copies of specific DNA segments quickly and accurately. These are needed for various experiments and procedures in molecular biology, forensic analysis (DNA fingerprinting), evolutionary biology (to amplify DNA fragments found in ancient specimens), and medicine (to diagnose genetic disease or detect low viral counts). Invented by Kary Mullis, PCR requires a DNA template (as little as one molecule) to copy, nucleotides to build the copies, and the enzyme DNA polymerase to catalyze the formation of bonds between the nucleotide monomers. Each three-step cycle (separating the two strands of the DNA double helix, marking the ends of the segment to be copied, and catalyzing the formation of bonds), which takes only minutes to complete, doubles the number of DNA strands present in the reaction medium. Repetition of this cycle many times results in an exponential increase in the amount of DNA

PCR

Hyphenation

polymerase Chain re·ac·tion

Turkish pronunciation

pälîmıreys çeyn riäkşın

Pronunciation

/ˈpäləmərˌās ˈʧān rēˈaksʜən/ /ˈpɑːlɪmɜrˌeɪs ˈʧeɪn riːˈækʃən/