Expressed sequence tag ESTs are usually short (300-500 hp) single reads from mRNA (cDNA) which are usually produced in large numbers They represent a snapshot of what is expressed in a given tissue, and/or at a given developmental stage They represent tags (some coding, others not) of expression for a given CDNA library (See: Expressed Sequence Tag)
Expressed sequence tags These are single-pass sequences of cDNA clones Databases of EST sequences are highly redundant, but quite useful for gene identification There are many efforts to cluster EST sequences to remove the redundancy and low quality sequences EST clusters in UniGene Gene indexes at TIGR references: Adams MD et al (1991) Complementary DNA sequencing: expressed sequence tags and human genome project Science 252(5013): 1651-6 Marra M et al (1999) An encyclopedia of mouse genes Nat Genet 21(2): 191-4
(Erhard Seminars Training, est system): Mystical "human potential" system founded in 1971 by Scientologist Werner Erhard (born John Paul Rosenberg in 1935) Est included "consciousness projection" and, in 1985, became The Forum The Latin word "est" means "it is " Est theory held that everyone is perfect deep down and, in a godlike fashion, creates his or her own reality
standard time in the 5th time zone west of Greenwich, reckoned at the 75th meridian; used in the eastern United States
Expressed Sequence Tag A partial sequence of a randomly chosen cDNA, obtained from the results of a single DNA sequencing reaction ESTs are used to identify transcribed regions in genomic sequence, to characterize patterns of gene expression in the tissue that was the source of the cDNA and as markers for genetic mapping