flow cytometry

listen to the pronunciation of flow cytometry
الإنجليزية - التركية
(Tıp) flow sitometre
الإنجليزية - الإنجليزية
A technique used to sort and classify cells by using fluorescent markers on their surface
The analysis of biological material by detection of properties of cells or subcellular fractions using a combination of fluorescence and a laser beam This makes it possible to sort cells or subcellular fractions for further analysis
A method of measuring physical and chemical attributes of cells or other biological particles by sensors, as they move by, one after the other, homogeneously suspended in fluid This rapid (about 10,000 particles per second) analytic method allows identification of important cell types, such as malignant cells, T cells and B cells This process also may utilize immunologic binding methods to further identify particular cell groups
(FC), is an emerging technique which holds great promise for the separation, classification and quantitation of blood cells and antibodies which affect blood cells Complex computerized instruments are used to pass a monocellular stream of cells, platelets or other microscopic particulate elements through a beam of laser light The cells are categorized first by size and then computer analyzed to sort the mixture of cellular elements into cell type by size In addition, monoclonal antibodies to specific cell surface markers are conjugated to fluorescent dyes and each cell displaying appropriate fluorescent light emission is counted Tabulation of counted data in conjunction with size analysis enables determination of relative percentages of each specific cellular subset for which monoclonal conjugates are utilized, even when the size of the cell is identical to other subset species
The measurement of cell activity on a singular basis
流式細胞儀, Analysis of biological material by detection of the light- absorbing or fluorescing properties of cells or subcellular fractions (i e , chromosomes) passing in a narrow stream through a laser beam An absorbance or fluorescence profile of the sample is produced Automated sorting devices, used to fractionate samples, sort successive droplets of the analyzed stream into different fractions depending on the fluorescence emitted by each droplet
a method to measure various biological parameters of cells by first treating them with light absorbing or fluorescing compounds and then passing them through a narrow fluid stream that is interrogated with a laser beam
Analysis of biological material by detection of the light-absorbing or fluorescing properties of cells or subcellular fractions (i e , chromosomes) passing in a narrow stream through a laser beam An absorbance or fluorescence profile of the sample is produced Automated sorting devices, used to fractionate samples, sort successive droplets of the analyzed stream into different fractions depending on the fluorescence emitted by each droplet
a procedure that uses a laser-powered instrument to measure the amount of DNA in cells It may be used to evaluate the risk of the recurrence of some cancers An abnormal amount of DNA may indicate a greater risk of recurrence [ Top ]
Flow cytometry is a method used to count cells (especially blood cells), using a glass chamber of known volume
The analysis and sorting of cells that have been reacted with fluorescent probes The sorting process is based on detection of fluorescence emitted by single cells within droplets